Short Communication Determination of Serum Albumin with Tribromoarsenazo by Spectrophotometry
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چکیده
The reaction of tribromoarsenazo(TB-ASA) with serum albumin in the presence of emulgent OP was studied by spectrophotometry. In a Britton-Robinson buffer solution at pH 2.9, tribromoarsenazo and bovine serum albumin can immediately form a red compound in the presence of emulgent OP with a maximum absorption wavelength at 354 nm. The presence of emulgent OP can increase the reaction sensitivity and the compound stability. The molar absorptivity of the compound is ε 354 nm = 6.13 x 10 5 M -1 ·cm -1 . Beer’s law is obeyed over the range of 5.0-75.0 mg·L -1 for bovine serum albumin. The present method was applied to the determination of the total proteins in human serums with satisfactory results.
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تاریخ انتشار 2008